【摘要】 目的建立一种新的快速诊断巨细胞病毒(CMV)感染的方法并初步用于肾移植受者。方法 采用抗CMV抗原的单克隆抗体，运用免疫组化链菌素-生物素标记法对外周血白细胞核中CMV抗原(即刻早期抗原和早期抗原)进行染色，诊断CMV感染。结果共检测肾移植受者72例，CMV活动性感染率为47%(34/72)，CMV病发病为18%(13/72)。CMV抗原阳性细胞数在CMV活动性感染者中平均为11.5/5万WBC，在CMV病者为20.5±17.8/5万WBC。同时检测10名健康人，结果均为阴性。结论该法具有快速、简便、成本低等优点，且能达到定量检测的目的，可用于临床对CMV感染的早期快速诊断。
【Abstract】Objective to establish a new method for the diagnosis of human cytomegalovirus(HCMV) infection and disease in renal transplant recipients.Methods labeled streptavidin biotin (LSAB)method with CMV monoclonal antibody was used to detect CMV antigen in peripher、al blood leukocytes.Results CMV antigen positive cells were counted in every5.0×104 wbc. 72 renal transplant recipients wereinvestigated. The rate of CMV active infection was 47% (34/72) and the average count of CMV antigen positive cells was 11.5/5.0×104 WBC. The incidence of CMV disease was 18% (13/72) with a CMV antigen value of 20.5±17.8/5.0×104 WBC. 10 healthy people were also studied and the results of CMV antigen were negative.Conclusions This method provides a rapid, accurate&nb sp;and low cost approach to the diagnosis of CMV infection and can be quantified easily.
【Key words】 cytomegalovirus infections Antigens, viral Kidney transplantation Labeled streptavidin biotin method
对象与方法
一、研究对象
检测1996年2月～1997年3月在本院接受肾移植的受者72例，其中男52例，女20例，年龄26～65岁，平均42.5岁。所有受者术后均用环孢素A＋强的松＋硫唑嘌呤(CSA＋pred＋Aza)三联免疫抑制方案。
二、设备及试剂
普通光学显微镜； CMV单克隆抗体；LSAB试剂盒(购自丹麦DAKO公司，批号为K9002)；Tris碱。